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LAMP1 Rabbit pAb (A23253)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - LAMP1 Rabbit pAb (A23253)

Western blot analysis of various lysates, using LAMP1 Rabbit pAb (A23253) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - LAMP1 Rabbit pAb (A23253)

Immunofluorescence analysis of NIH/3T3 cells using LAMP1 Rabbit pAb (A23253) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLAMP1 Rabbit pAb
Catalog No.A23253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein domain specific binding activity. Involved in protein stabilization. Located in several cellular components, including cytoplasmic vesicle; sarcolemma; and vacuole. Is expressed in several structures, including 1-cell stage embryo; central nervous system; craniocervical region bone; extraembryonic component; and gut. Orthologous to human LAMP1 (lysosomal associated membrane protein 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 208-363 of mouse LAMP1 (NP_001304282.1).
SequencePTVSKYNVTGNNGTCLLASMALQLNITYLKKDNKTVTRAFNISPNDTSSGSCGINLVTLKVENKNRALELQFGMNASSSLFFLQGVRLNMTLPDALVPTFSISNHSLKALQATVGNSYKCNTEEHIFVSKMLSLNVFSVQVQAFKVDSDRFGSVEE
Gene ID
Swiss Prot
SynonymsP2B; Perk; LGP-A; CD107a; Lamp-1; LGP-120; LAMP1
Calculated MW44kDa
Observed MW90-120kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesC2C12, NIH/3T3
Cellular location
Customer validation

Other(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LAMP1 Rabbit pAb (A23253)}

    Western blot - LAMP1 Rabbit pAb (A23253)

    Western blot analysis of various lysates, using LAMP1 Rabbit pAb (A23253) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - LAMP1 Rabbit pAb (A23253)}

    Immunofluorescence - LAMP1 Rabbit pAb (A23253)

    Immunofluorescence analysis of NIH/3T3 cells using LAMP1 Rabbit pAb (A23253) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23253? Please let us know so that we can cite the reference in this datasheet.

    항체 (6)

    Secondary Antibodies (24)