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LCK Rabbit pAb (A2177)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LCK Rabbit pAb (A2177)

Western blot analysis of lysates from Mouse thymus, using LCK Rabbit pAb (A2177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - LCK Rabbit pAb (A2177)

Western blot analysis of various lysates using LCK Rabbit pAb (A2177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded rat spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameLCK Rabbit pAb
Catalog No.A2177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LCK (NP_005347.3).
SequenceMGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEV
Gene ID
Swiss Prot
SynonymsLSK; YT16; IMD22; p56lck; pp58lck; LCK
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, Mouse lung, Mouse thymus
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - LCK Rabbit pAb (A2177)}

Western blot - LCK Rabbit pAb (A2177)

Western blot analysis of lysates from Mouse thymus, using LCK Rabbit pAb (A2177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - LCK Rabbit pAb (A2177)}

Western blot - LCK Rabbit pAb (A2177)

Western blot analysis of various lysates using LCK Rabbit pAb (A2177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)}

Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded rat spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)}

Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LCK Rabbit pAb (A2177)}

Immunohistochemistry - LCK Rabbit pAb (A2177)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using LCK Rabbit pAb (A2177) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2177? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)