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Phospho-Lck-Y505 Rabbit pAb (AP0285)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Phospho-Lck-Y505 Rabbit pAb (AP0285) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes or treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-Lck-Y505 Rabbit pAb (AP0285)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg Phospho-Lck-Y505 pAb (AP0285). Western blot was performed from the immunoprecipitate using Phospho-Lck-Y505 pAb (AP0285) at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.

Overview

Product name	Phospho-Lck-Y505 Rabbit pAb
Catalog No.	AP0285
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y505 of human Lck (NP_001036236.1).
Sequence	GQYQP
Gene ID
Swiss Prot
Synonyms	LSK; YT16; IMD22; p56lck; pp58lck; Phospho-Lck-Y505
Calculated MW	58kDa
Observed MW	56kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	Jurkat treated by Hydrogen Peroxide or Pervanadate
Cellular location	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor.