제품 > 항체 > 인산화 특이적 항체

Phospho-LCK-Y394 Rabbit pAb (AP0182)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit pAb (AP0182) at 1:1000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from Jurkat cells, using Phospho-LCK-Y394 Rabbit pAb (AP0182) at 1:1000 dilution. Jurkat cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-LCK-Y394 Rabbit pAb (AP0182)

Immunofluorescence analysis of Raji pervanadate and Raji cells using Phospho-LCK-Y394 Rabbit pAb(AP0182) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-LCK-Y394 Rabbit pAb
Catalog No.	AP0182
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y394 of human LCK (NP_005347.3).
Sequence	NEYTA
Gene ID
Swiss Prot
Synonyms	LSK; YT16; IMD22; p56lck; pp58lck; Phospho-LCK-Y394
Calculated MW	58kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by Pervanadate, Jurkat
Cellular location	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor.
Customer validation	WB(Homo sapiens)