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MAP2 Rabbit mAb (A22205)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MAP2 Rabbit mAb (A22205)

Western blot analysis of lysates from SH-SY5Y cells, using MAP2 Rabbit mAb (A22205) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MAP2 Rabbit mAb (A22205)

Confocal imaging of paraffin-embedded Rat brain using MAP2 Rabbit mAb (A22205, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MAP2 Rabbit mAb (A22205)

Confocal imaging of paraffin-embedded Mouse brain using MAP2 Rabbit mAb (A22205, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameMAP2 Rabbit mAb
Catalog No.A22205
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56279
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human MAP2 (NP_002365.3).
SequenceMADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKD
Gene ID
Swiss Prot
SynonymsMAP-2; MAP2A; MAP2B; MAP2C; MAP2
Calculated MW200kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationCytoplasm, Cytoskeleton.
Customer validation

IF(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - MAP2 Rabbit mAb (A22205)}

Western blot - MAP2 Rabbit mAb (A22205)

Western blot analysis of lysates from SH-SY5Y cells, using MAP2 Rabbit mAb (A22205) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)}

Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)}

Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit mAb (A22205)}

Immunohistochemistry - MAP2 Rabbit mAb (A22205)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using MAP2 Rabbit mAb (A22205) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MAP2 Rabbit mAb (A22205)}

Immunofluorescence - MAP2 Rabbit mAb (A22205)

Confocal imaging of paraffin-embedded Rat brain using MAP2 Rabbit mAb (A22205, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MAP2 Rabbit mAb (A22205)}

Immunofluorescence - MAP2 Rabbit mAb (A22205)

Confocal imaging of paraffin-embedded Mouse brain using MAP2 Rabbit mAb (A22205, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A22205? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)