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MAP2 Rabbit pAb (A0453)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MAP2 Rabbit pAb (A0453)

Western blot analysis of lysates from Mouse brain, using MAP2 Rabbit pAb (A0453) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A0453)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A0453) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A0453)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A0453) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - MAP2 Rabbit pAb (A0453)

Immunofluorescence analysis of paraffin-embedded mouse brain using MAP2 Rabbit pAb (A0453) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MAP2 Rabbit pAb (A0453)

Immunofluorescence analysis of paraffin-embedded rat brain using MAP2 Rabbit pAb (A0453) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMAP2 Rabbit pAb
Catalog No.A0453
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human MAP2 (NP_002365.3).
SequenceMADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKD
Gene ID
Swiss Prot
SynonymsMAP-2; MAP2A; MAP2B; MAP2C; MAP2
Calculated MW200kDa
Observed MW220-280kd(MAP2A、MAP2B)
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationCytoplasm, cytoskeleton.
Customer validation

WB(Homo sapiens, Mus musculus)

Other(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - MAP2 Rabbit pAb (A0453)}

Western blot - MAP2 Rabbit pAb (A0453)

Western blot analysis of lysates from Mouse brain, using MAP2 Rabbit pAb (A0453) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A0453)}

Immunohistochemistry - MAP2 Rabbit pAb (A0453)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A0453) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A0453)}

Immunohistochemistry - MAP2 Rabbit pAb (A0453)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A0453) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - MAP2 Rabbit pAb (A0453)}

Immunofluorescence - MAP2 Rabbit pAb (A0453)

Immunofluorescence analysis of paraffin-embedded mouse brain using MAP2 Rabbit pAb (A0453) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MAP2 Rabbit pAb (A0453)}

Immunofluorescence - MAP2 Rabbit pAb (A0453)

Immunofluorescence analysis of paraffin-embedded rat brain using MAP2 Rabbit pAb (A0453) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)