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MMP14/MT1-MMP Rabbit pAb (A2549)

Review (1)Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MMP14/MT1-MMP Rabbit pAb (A2549)

Western blot analysis of lysates from Rat spleen using MMP14/MT1-MMP Rabbit pAb (A2549) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of HeLa cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of NIH/3T3 cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of PC-12 cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for MMP14/MT1-MMP Rabbit pAb(A2549)

Overview

Product nameMMP14/MT1-MMP Rabbit pAb
Catalog No.A2549
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 75-174 of human MMP14/MMP14/MT1-MMP (NP_004986.1).
SequenceQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQ
Gene ID
Swiss Prot
SynonymsMMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP14/MT1-MMP
Calculated MW66kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat spleen
Cellular locationCytoplasm, Melanosome, Membrane, Single-pass type I membrane protein.
Customer validation

(MDA-MB-231)

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens)

IHC(Homo sapiens)

Documents

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ABclonal:Western blot - MMP14/MT1-MMP Rabbit pAb (A2549)}

Western blot - MMP14/MT1-MMP Rabbit pAb (A2549)

Western blot analysis of lysates from Rat spleen using MMP14/MT1-MMP Rabbit pAb (A2549) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunohistochemistry - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of HeLa cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of NIH/3T3 cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)}

Immunofluorescence - MMP14/MT1-MMP Rabbit pAb (A2549)

Immunofluorescence analysis of PC-12 cells using MMP14/MT1-MMP Rabbit pAb (A2549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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