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PHLPP1 Rabbit pAb (A9542)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PHLPP1 Rabbit pAb (A9542)

Western blot analysis of various lysates using PHLPP1 Rabbit pAb (A9542) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PHLPP1 Rabbit pAb (A9542)

Immunofluorescence analysis of U-2 OS cells using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePHLPP1 Rabbit pAb
Catalog No.A9542
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1160-1428 of human PHLPP1 (NP_919431.2).
SequenceDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDS
Gene ID
Swiss Prot
SynonymsSCOP; PHLPP; PPM3A; PLEKHE1; PHLPP1
Calculated MW185kDa
Observed MW185kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, SH-SY5Y, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular locationCell membrane, Cytoplasm, Membrane, Nucleus, Peripheral membrane protein.
Customer validation

WB(Homo sapiens,Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - PHLPP1 Rabbit pAb (A9542)}

Western blot - PHLPP1 Rabbit pAb (A9542)

Western blot analysis of various lysates using PHLPP1 Rabbit pAb (A9542) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)}

Immunohistochemistry - PHLPP1 Rabbit pAb (A9542)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - PHLPP1 Rabbit pAb (A9542)}

Immunofluorescence - PHLPP1 Rabbit pAb (A9542)

Immunofluorescence analysis of U-2 OS cells using PHLPP1 Rabbit pAb (A9542) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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