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Phospho-ACC1-S79 Rabbit pAb (AP0298)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from A-431 cells, using Phospho-ACC1-S79 Rabbit pAb (AP0298) at 1:700 dilution. A-431 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Western blot analysis of lysates from RAW264.7 cells, using Phospho-ACC1-S79 Rabbit pAb (AP0298) at 1:700 dilution. RAW264. 7 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	Phospho-ACC1-S79 Rabbit pAb
Catalog No.	AP0298
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S79 of human ACC1 (NP_942133.1).
Sequence	SMSGL
Gene ID
Swiss Prot
Synonyms	ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha; Phospho-ACC1-S79
Calculated MW	266kDa
Observed MW	280kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431, RAW264.7
Cellular location	Cytoplasm.
Customer validation	WB(Rattus norvegicus, Mus musculus, Sus scrofa, Homo sapiens, Bos taurus) IHC(Homo sapiens) IHC(Homo sapiens)

Documents

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Research Area

Phospho-ACC1-S79 Rabbit pAb images

Western blot - Phospho-ACC1-S79 Rabbit pAb (AP0298)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Bioorganic & Medicinal Chemistry

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

29128163

Title:

Depsidone and xanthones from Garcinia xanthochymus with hypoglycemic activity and the mechanism of promoting glucose uptake in L6 myotubes

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Annals of Translational Medicine

Application:

IF:

Species:

Mus musculus

PMID:

32042730

Title:

The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

31991934

Title:

Sesamol Alleviates Obesity-Related Hepatic Steatosis via Activating Hepatic PKA Pathway

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Viruses

Application:

IF:

4.7

Species:

Sus scrofa

PMID:

31835577

Title:

Fatty Acids Regulate Porcine Reproductive and Respiratory Syndrome Virus Infection via the AMPK-ACC1 Signaling Pathway

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Cardiovascular Diagnosis and Therapy

Application:

IF:

2.1

Species:

Mus musculus

PMID:

32420095

Title:

Ginsenoside Rg3 protects heart against isoproterenol-induced myocardial infarction by activating AMPK mediated autophagy.

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Stem Cell Res Ther

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

33579362

Title:

Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Exp Cell Res

Application:

IF:

3.3

Species:

Mus musculus

PMID:

33675805

Title:

Adipose-specific knockout of Protein Kinase D1 suppresses de novo lipogenesis in mice via SREBP1c-dependent signaling

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.2

Species:

Mus musculus

PMID:

33785339

Title:

A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

9.1

Species:

Mus musculus

PMID:

36455814

Title:

Secreted MUP1 that reduced under ER stress attenuates ER stress induced insulin resistance through suppressing protein synthesis in hepatocytes

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular and cell biology of lipids

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

36690321

Title:

Zeaxanthin prevents ferroptosis by promoting mitochondrial function and inhibiting the p53 pathway in free fatty acid-induced HepG2 cells

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

36913987

Title:

Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive …

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Cancer letters

Application:

IHC

IF:

9.1

Species:

Homo sapiens

PMID:

37778683

Title:

Glucose restriction induces AMPK-SIRT1-mediated circadian clock gene Per expression and delays NSCLC progression

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

2.8

Species:

Homo sapiens

PMID:

37934902

Title:

Maackiain suppresses the development of cervical cancer via AMPK priming autophagy

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Journal of dairy science

Application:

IF:

3.7

Species:

Bos taurus

PMID:

37977448

Title:

Evaluation of hepatic AMP-activated protein kinase (AMPK), mechanistic target of rapamycin kinase complex 1 (mTORC1) and autophagy-lysosomal pathway

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Rattus norvegicus

PMID:

37338809

Title:

L-theanine attenuates heat stress-induced proteotoxicity and alterations in carbohydrate and lipid metabolism via heat shock factor 1

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Nature communications

Application:

WB, IHC

IF:

16.6

Species:

Homo sapiens

PMID:

36433955

Title:

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis - PMC

References for Phospho-ACC1-S79 Rabbit pAb (AP0298)

Product:

Phospho-ACC1-S79 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

35795562

Title:

Multi-Omics Integration Analysis Identifies Lipid Disorder of a Non-Alcoholic Fatty Liver Disease (NAFLD) Mouse Model Improved by Zexie-Baizhu Decoction - PMC