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Phospho-ATF2-T71 Rabbit pAb (AP0020)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-ATF2-T71 Rabbit pAb (AP0020) at 1:1000 dilution. HeLa cells were treated by Anisomycin (25μg/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25μg/mL) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-ATF2-T71 Rabbit pAb (AP0020)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using Phospho-ATF2-T71 Rabbit pAb (AP0020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-ATF2-T71 Rabbit pAb (AP0020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-ATF2-T71 Rabbit pAb (AP0020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	Phospho-ATF2-T71 Rabbit pAb
Catalog No.	AP0020
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T71 of human ATF2 (NP_001871.2).
Sequence	TPTPT
Gene ID
Swiss Prot
Synonyms	HB16; CREB2; TREB7; CREB-2; CRE-BP1; Phospho-ATF2-T71
Calculated MW	55kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa treated by Anisomycin, NIH/3T3 treated by Anisomycin
Cellular location	Cytoplasm, Mitochondrion outer membrane, Nucleus
Customer validation	WB(Mus musculus, Homo sapiens)