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ATF2 Rabbit mAb (A22718)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using ATF2 Rabbit mAb (A22718) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ATF2 Rabbit mAb (A22718)

Immunohistochemistry analysis of ATF2 in paraffin-embedded Human colon tissue using ATF2 Rabbit mAb (A22718) at a dilution of 1:500 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATF2 in paraffin-embedded Human lung cancer tissue using ATF2 Rabbit mAb (A22718) at a dilution of 1:500 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATF2 in paraffin-embedded Mouse brain tissue using ATF2 Rabbit mAb (A22718) at a dilution of 1:500 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATF2 in paraffin-embedded Mouse testis tissue using ATF2 Rabbit mAb (A22718) at a dilution of 1:500 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATF2 in paraffin-embedded Rat kidney tissue using ATF2 Rabbit mAb (A22718) at a dilution of 1:500 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - ATF2 Rabbit mAb (A22718)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg ATF2 antibody (A22718). Western blot was performed from the immunoprecipitate using ATF2 antibody (A22718) at a dilution of 1:60000.

Overview

Product name	ATF2 Rabbit mAb
Catalog No.	A22718
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55522

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human ATF2 (NP_001871.2).
Sequence	MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIVPVPGPFPLLLHLPNGQTMPVAIPASITSSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQTQSTS
Gene ID
Swiss Prot
Synonyms	HB16; CREB2; TREB7; CREB-2; CRE-BP1; ATF2
Calculated MW	55kDa
Observed MW	74kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:70000 IHC-P 1:100 - 1:500 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	COLO 205(Negative control), HeLa, C2C12, Rat thymus
Cellular location	Cytoplasm, Mitochondrion outer membrane, Nucleus
Customer validation	WB(Homo sapiens)