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Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T69/71 Rabbit pAb (A2155). 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namePhospho-ATF2-T69/71 Rabbit pAb
Catalog No.AP0913
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
ImmunogenA synthetic phosphorylated peptide around T69 & T71 of human ATF2 (NP_001871.2?).
SequenceQTPTP
Gene ID
Swiss Prot
SynonymsHB16; CREB2; TREB7; CREB-2; CRE-BP1; Phospho-ATF2-T69/71
Calculated MW55kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T treated by UV
Cellular locationCytoplasm, Mitochondrion outer membrane, Nucleus.

Documents

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ABclonal:Western blot - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)}

Western blot - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T69/71 Rabbit pAb (A2155). 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)}

Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)}

Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)}

Immunohistochemistry - Phospho-ATF2-T69/71 Rabbit pAb (AP0913)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-ATF2-T69/71 Rabbit pAb (AP0913) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)