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Phospho-ATF2-T71 Rabbit mAb (AP1051)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Phospho-ATF2-T71 Rabbit mAb (AP1051)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (AP1051) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product namePhospho-ATF2-T71 Rabbit mAb
Catalog No.AP1051
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1598
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
ImmunogenA synthetic phosphorylated peptide around T71 of human ATF2 (P15336).
SequenceTPTPT
Gene ID
Swiss Prot
SynonymsHB16; CREB2; TREB7; CREB-2; CRE-BP1; Phospho-ATF2-T71
Calculated MW55kDa
Observed MW70kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by Anisomycin
Cellular locationCytoplasm, Mitochondrion outer membrane, Nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-ATF2-T71 Rabbit mAb (AP1051)}

Western blot - Phospho-ATF2-T71 Rabbit mAb (AP1051)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (AP1051) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP1051? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)