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Phospho-GSK3β-S9 Rabbit pAb (AP0358)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of extracts from 293 cells, using Phospho-GSK3β-S9 antibody (AP0358).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Review

Overview

Product name	Phospho-GSK3β-S9 Rabbit pAb
Catalog No.	AP0358
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S9 of human GSK3β
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	GSK3B; gsk-3β
Calculated MW	47kDa
Observed MW	47kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:200 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293
Cellular location	Cell membrane, Cytoplasm, Nucleus
Customer validation	(Human chronic myeloid leukemia cells) WB(Homo sapiens, Mus musculus) IHC(Mus musculus) IF(Homo sapiens)