제품 > 항체 > 인산화 특이적 항체

Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Western blot analysis of lysates from PC-12 cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	Phospho-HSP27/HSPB1-S82 Rabbit mAb
Catalog No.	AP1031
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1576

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S82 of human HSP27/HSPB1HSPB1 (P04792).
Sequence	QLSSG
Gene ID
Swiss Prot
Synonyms	CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; Phospho-HSP27/HSPB1-S82
Calculated MW	23kDa
Observed MW	27kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by UV, PC-12, PC-12 treated by UV
Cellular location	Cytoplasm, Nucleus, Cytoskeleton, Spindle.
Customer validation	WB(Mus musculus) IP(Mus musculus)