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Phospho-HSP27/HSPB1-S82 Rabbit pAb (AP0041)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit pAb (AP0041) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 pAb (AP0041) at 1:1000 dilution or HSP27/HSPB1 antibody (A16332). HeLa cells were treated by UV at room temperature for 15-30 minutes.HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - Phospho-HSP27/HSPB1-S82 Rabbit pAb (AP0041)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-HSP27/HSPB1-S82 pAb (AP0041). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S82 pAb (AP0041) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

Review

Overview

Product name	Phospho-HSP27/HSPB1-S82 Rabbit pAb
Catalog No.	AP0041
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S82 of human HSP27/HSPB1 (NP_001531.1).
Sequence	QLSSG
Gene ID
Swiss Prot
Synonyms	CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; Phospho-HSP27/HSPB1-S82
Calculated MW	23kDa
Observed MW	28kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa treated by EGF, HeLa treated by UV, HeLa treated by Anisomycin
Cellular location	Cytoplasm, Nucleus, cytoskeleton, spindle.
Customer validation	(HepG2) WB(Toxoplasma gondii, Homo sapiens, Rattus norvegicus)