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HSP27/HSPB1 Rabbit pAb (A0240)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Hep G2 cells using HSP27/HSPB1 Rabbit pAb (A0240) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from Rat lung cells using HSP27/HSPB1 Rabbit pAb (A0240) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from HeLa cells using HSP27/HSPB1 Rabbit pAb (A0240) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot analysis of lysates from Mouse lung using HSP27/HSPB1 Rabbit pAb (A0240) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HSP27/HSPB1 Rabbit pAb (A0240)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HSP27/HSPB1 Rabbit pAb (A0240) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review

Overview

Product name	HSP27/HSPB1 Rabbit pAb
Catalog No.	A0240
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human HSP27/HSPB1 (NP_001531.1).
Sequence	MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLD
Gene ID
Swiss Prot
Synonyms	CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; HSP27/HSPB1
Calculated MW	23kDa
Observed MW	25kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:1000 - 1:5000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HepG2, HeLa, mouse lung, rat lung
Cellular location	Cytoplasm, Nucleus, cytoskeleton, spindle.
Customer validation	(Mouse N2a,Mouse kidney, HepG2, HeLa) WB(Rattus norvegicus, Homo sapiens, Toxoplasma gondii, Mus musculus) Co-IP(Homo sapiens) IHC(Homo sapiens, Mus musculus) IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

HSP27/HSPB1 Rabbit pAb images

Western blot - HSP27/HSPB1 Rabbit pAb (A0240)

Immunohistochemistry - HSP27/HSPB1 Rabbit pAb (A0240)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Basic Research in Cardiology

Application:

IF:

17.165

Species:

Rattus norvegicus

PMID:

28534118

Title:

Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Apoptosis : an International Journal on Programmed Cell Death

Application:

IF:

4.677

Species:

Homo sapiens

PMID:

30284149

Title:

Inhibition of the ubiquitination of HSF1 by FBXW7 protects the intestine against ischemia–reperfusion injury

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Biochimica et biophysica acta. Proteins and proteomics

Application:

IF:

3.036

Species:

Toxoplasma gondii

PMID:

30576742

Title:

iTRAQ-based phosphoproteomic analysis reveals host cell's specific responses to Toxoplasma gondii at the phases of invasion and prior to egress.

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

30455644

Title:

Salvianolic Acid A Attenuates Endoplasmic Reticulum Stress and Protects Against Cholestasis-Induced Liver Fibrosis via the SIRT1/HSF1 Pathway.

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.479

Species:

Homo sapiens

PMID:

26646625

Title:

Gold-nanorods-siRNA nanoplex for improved photothermal therapy by gene silencing

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Food Chem Toxicol

Application:

IF:

6.023

Species:

Homo sapiens

PMID:

32619556

Title:

Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

Co-IP

IF:

6.69

Species:

Homo sapiens

PMID:

34581912

Title:

MS4A3-HSP27 target pathway reveals potential for haematopoietic disorder treatment in alimentary toxic aleukia

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Proteomics

Application:

IF:

3.984

Species:

Mus musculus

PMID:

34951099

Title:

Quantitative proteomics revealed new functions of ALKBH4

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

British journal of cancer

Application:

IF:

9.07

Species:

Homo sapiens

PMID:

35896815

Title:

Thermal stress involved in TRPV2 promotes tumorigenesis through the pathways of HSP70/27 and PI3K/Akt/mTOR in esophageal squamous cell carcinoma

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Cell reports. Medicine

Application:

IHC

IF:

Species:

Homo sapiens, Mus musculus

PMID:

35243415

Title:

SU086, an inhibitor of HSP90, impairs glycolysis and represents a treatment strategy for advanced prostate cancer

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

Aging

Application:

WB, IF

IF:

5.95

Species:

Homo sapiens

PMID:

36107005

Title:

Identification of alanine aminotransferase 1 interaction network via iTRAQ-based proteomics in alternating migration, invasion, proliferation and apoptosis of HepG2 cells - PMC

References for HSP27/HSPB1 Rabbit pAb (A0240)

Product:

HSP27/HSPB1 Rabbit pAb

Journal:

BMC pulmonary medicine

Application:

IF:

3.32

Species:

Mus musculus

PMID:

30482200