Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (AP1142) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (AP1142) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
| Product name | Phospho-JunD-S255 Rabbit mAb |
|---|---|
| Catalog No. | AP1142 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1603 |
| Immunogen | A synthetic phosphorylated peptide around S255 of human JunD (P17535). |
|---|---|
| Sequence | GESPP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AP-1; Phospho-JunD-S255 |
| Calculated MW | 35kDa |
| Observed MW | 38kDa/42kDa |
| Reactivity | Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | NIH/3T3 treated by Anisomycin, NIH/3T3 treated by UV |
| Cellular location | Nucleus. |
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