Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of extracts of HeLa cells, using Phospho-JunD-S255 antibody (AP1323) at1:2000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
| Product name | Phospho-JunD-S255 Rabbit mAb |
|---|---|
| Catalog No. | AP1323 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC54895 |
| Immunogen | A synthetic phosphorylated peptide around S255 of human Phospho-JunD-S255 (NP_005345.3). |
|---|---|
| Sequence | GESPP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AP-1 |
| Calculated MW | 35kDa |
| Observed MW | 38kDa/42kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa |
| Cellular location | Nucleus |
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