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Phospho-MDM2-S166 Rabbit pAb (AP0073)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (AP0073). 293 cells were treated by Hydroxyurea (4mM) for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunofluorescence - Phospho-MDM2-S166 Rabbit pAb (AP0073)

Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using Phospho-MDM2-S166 Rabbit pAb (AP0073) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Review

Overview

Product name	Phospho-MDM2-S166 Rabbit pAb
Catalog No.	AP0073
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).
Sequence	RAISET
Gene ID
Swiss Prot
Synonyms	HDMX; LSKB; hdm2; ACTFS; Phospho-MDM2-S166
Calculated MW	55kDa
Observed MW	95kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293
Cellular location	Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Customer validation	(Adenocarcinoma human alveolar basal epithelial cells) Co-IP(Mus musculus)