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Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0426)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0426) at 1:1000 dilution. NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	Phospho-PDK1/PDPK1-S241 Rabbit pAb
Catalog No.	AP0426
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S241 of human PDK1/PDPK1
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	PDK1; PDPK2; PDPK2P; PRO0461; Phospho-PDK1/PDPK1-S241
Calculated MW	63kDa
Observed MW	58-68kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:100 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3
Cellular location	Cell junction, Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, focal adhesion.
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Homo sapiens, Mus musculus)