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Phospho-SRC Family-Y416 Rabbit pAb (AP0511)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH3T3 cells, using Phospho-SRC Family-Y416 Rabbit pAb (AP0511).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-SRC Family-Y416 Rabbit pAb
Catalog No.	AP0511
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y416 of human SRC FamilySRC (NP_005408.1).
Sequence	NEYTA
Gene ID
Swiss Prot
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src; SRC; Phospho-SRC Family-Y416
Calculated MW	54kDa/60kDa/40kDa/58kDa/61kDa
Observed MW	61kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3
Cellular location	cytosol, dendrite, endosome, glial cell projection, mitochondrion, nucleus, perikaryon, perinuclear endoplasmic reticulum, perinuclear region of cytoplasm, plasma membrane, postsynaptic density, Schaffer collateral - CA1 synapse.
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Mus musculus) IF(Mus musculus)