Review (1)Publications (12) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HeLa cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution. HeLa cells were treated by IFN-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT1-Y701 (AP0135) at 1:630 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
| Product name | Phospho-STAT1-Y701 Rabbit pAb |
|---|---|
| Catalog No. | AP0135 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around Y701 of human STAT1 (NP_009330.1). |
|---|---|
| Sequence | TGYIK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; Phospho-STAT1-Y701 |
| Calculated MW | 87kDa |
| Observed MW | 84kDa/91kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | HeLa treated by IFN-α, NIH/3T3 treated by TNF-α |
| Cellular location | Cytoplasm, Nucleus. |
| Customer validation | (Human chronic myeloid leukemia cells) WB(Mus musculus, Homo sapiens, Sus scrofa) IP(Mus musculus) |
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