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Phospho-STAT5A-S780 Rabbit pAb (AP0139)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-STAT5A-S780 Rabbit pAb (AP0139)

Western blot analysis of lysates from K562 cells using Phospho-STAT5A-S780 Rabbit pAb (AP0139).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-STAT5A-S780 Rabbit pAb
Catalog No.AP0139
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S780 of human STAT5A
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMGF; STAT5; Phospho-STAT5A-S780
Calculated MW91kDa
Observed MW91kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesK-562
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - Phospho-STAT5A-S780 Rabbit pAb (AP0139)}

Western blot - Phospho-STAT5A-S780 Rabbit pAb (AP0139)

Western blot analysis of lysates from K562 cells using Phospho-STAT5A-S780 Rabbit pAb (AP0139).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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