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Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Western blot analysis of lysates from TF-1 cells, using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at 1:1000 dilution. TF-1 cells were treated by GM-CSF (25 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human liver using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product namePhospho-STAT5A-Y694 Rabbit mAb
Catalog No.AP0758
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0176
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y694 of human Stat5 (P42229).
SequenceDGYVK
Gene ID
Swiss Prot
SynonymsMGF; STAT5; Phospho-STAT5A-Y694
Calculated MW91kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesTF-1 treated by GM-CSF
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus, Homo sapiens, Other)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Western blot - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Western blot analysis of lysates from TF-1 cells, using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at 1:1000 dilution. TF-1 cells were treated by GM-CSF (25 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human liver using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)}

Immunohistochemistry - Phospho-STAT5A-Y694 Rabbit mAb (AP0758)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT5A-Y694 Rabbit mAb (AP0758) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)