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STAT5A Rabbit pAb (A7733)

Review (1)Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - STAT5A Rabbit pAb (A7733)

Western blot analysis of extracts of HeLa cells, using STAT5A antibody (A7733) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of C6 cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of NIH/3T3 cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of U2OS cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for STAT5A Rabbit pAb(A7733)

Overview

Product nameSTAT5A Rabbit pAb
Catalog No.A7733
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 635-794 of human STAT5A (NP_003143.2).
SequenceSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS
Gene ID
Swiss Prot
SynonymsMGF; STAT5
Calculated MW91kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

(U937)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

Documents

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ABclonal:Western blot - STAT5A Rabbit pAb (A7733)}

Western blot - STAT5A Rabbit pAb (A7733)

Western blot analysis of extracts of HeLa cells, using STAT5A antibody (A7733) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)}

Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of C6 cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)}

Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of NIH/3T3 cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT5A Rabbit pAb (A7733)}

Immunofluorescence - STAT5A Rabbit pAb (A7733)

Immunofluorescence analysis of U2OS cells using STAT5A Rabbit pAb (A7733) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)