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Phospho-Smad5-S463/S465 Rabbit mAb (AP1023)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (AP1023) at 1:1000 dilution or Smad5 antibody (A1947). Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product name	Phospho-Smad5-S463/S465 Rabbit mAb
Catalog No.	AP1023
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1568

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S463 & S465 of human Smad5 (Q99717).
Sequence	SSVS
Gene ID
Swiss Prot
Synonyms	DWFC; JV5-1; MADH5; Phospho-Smad5-S463/S465
Calculated MW	52kDa
Observed MW	52kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Hela treated by ATP
Cellular location	Cytoplasm, Nucleus.