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Phospho-Stathmin 1-S16 Rabbit pAb (AP0229)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-Stathmin 1-S16 Rabbit pAb (AP0229)

Western blot analysis of lysates from HeLa and C2C12 cells, using Phospho-Stathmin 1-S16 Rabbit pAb (AP0229) at 1:2000 dilution. HeLa and C2C12 cells were treated by Nocodazole (50ng/mL) for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product namePhospho-Stathmin 1-S16 Rabbit pAb
Catalog No.AP0229
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S16 of human Stathmin 1 (NP_001138926.1).
SequenceRASGQ
Gene ID
Swiss Prot
SynonymsLag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; Phospho-Stathmin 1-S16
Calculated MW17kDa
Observed MW15kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Nocodazol, C2C12 treated by Nocodazol
Cellular locationCytoplasm, cytoskeleton.

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ABclonal:Western blot - Phospho-Stathmin 1-S16 Rabbit pAb (AP0229)}

Western blot - Phospho-Stathmin 1-S16 Rabbit pAb (AP0229)

Western blot analysis of lysates from HeLa and C2C12 cells, using Phospho-Stathmin 1-S16 Rabbit pAb (AP0229) at 1:2000 dilution. HeLa and C2C12 cells were treated by Nocodazole (50ng/mL) for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (6)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)