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Phospho-Stathmin 1-S25 Rabbit mAb (AP1440)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from C6 cells, using Phospho-Stathmin 1-S25 Rabbit mAb (AP1440) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	Phospho-Stathmin 1-S25 Rabbit mAb
Catalog No.	AP1440
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62298

Background

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S25 of human Stathmin 1（NP_005554.1）.
Sequence	RASGQ
Gene ID
Swiss Prot
Synonyms	Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; Phospho-Stathmin 1-S25
Calculated MW	17kDa
Observed MW	17kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C6 treated by PMA
Cellular location	Cytoplasm, Cytoskeleton