Western blot - Stathmin 1 Rabbit mAb (A4379)
Western blot analysis of various lysates using Stathmin 1 Rabbit mAb (A4379) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Human colon using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Human placenta using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Human testis using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Rat colon using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Stathmin 1 Rabbit mAb (A4379)
Immunohistochemistry analysis of paraffin-embedded Rat spleen using Stathmin 1 Rabbit mAb (A4379) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - Stathmin 1 Rabbit mAb (A4379)
Confocal imaging of paraffin-embedded Rat brain using Stathmin 1 Rabbit mAb (A4379, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Immunofluorescence - Stathmin 1 Rabbit mAb (A4379)
Confocal imaging of paraffin-embedded Mouse brain using Stathmin 1 Rabbit mAb (A4379, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Immunofluorescence - Stathmin 1 Rabbit mAb (A4379)
Confocal imaging of HeLa cells using Stathmin 1 Rabbit mAb (A4379, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.