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Phospho-Syk-Y323 Rabbit pAb (AP0291)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Syk-Y323 Rabbit pAb (AP0291)

Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (AP0291).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-Syk-Y323 Rabbit pAb
Catalog No.AP0291
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y323 of human Syk
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsIMD82; p72-Syk; Phospho-Syk-Y323
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat
Cellular locationCell membrane, Cytoplasm, cytosol
ABclonal:Western blot - Phospho-Syk-Y323 Rabbit pAb (AP0291)}

Western blot - Phospho-Syk-Y323 Rabbit pAb (AP0291)

Western blot analysis of lysates from Jurkat cells using Phospho-Syk-Y323 Rabbit pAb (AP0291).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)