제품 > 항체 > 인산화 특이적 항체

Phospho-Tyrosine Hydroxylase-S40 Rabbit pAb (AP1109)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Rat

Western blot analysis of lysates from PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (AP1109) at 1:1000 dilution or Tyrosine Hydroxylase antibody (A0028). PC-12 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product name	Phospho-Tyrosine Hydroxylase-S40 Rabbit pAb
Catalog No.	AP1109
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S40 of human Tyrosine Hydroxylase (NP_000351.2?).
Sequence	RQSLI
Gene ID
Swiss Prot
Synonyms	TYH; DYT14; DYT5b; Phospho-Tyrosine Hydroxylase-S40
Calculated MW	59kDa
Observed MW	Refer to figures

Applications

Reactivity	Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	PC-12 treated by Forskolin
Cellular location	axon, cytoplasm, cytoplasmic side of plasma membrane, cytoplasmic vesicle, cytosol, dendrite, mitochondrion, neuron projection, nucleus, perikaryon, perinuclear region of cytoplasm, smooth endoplasmic reticulum, synaptic vesicle, terminal bouton.