Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (AP0736) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
| Product name | Phospho-ULK1-S757 Rabbit pAb |
|---|---|
| Catalog No. | AP0736 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_003556.1). |
|---|---|
| Sequence | TVGSP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ATG1; ATG1A; UNC51; hATG1; Unc51.1; Phospho-ULK1-S757 |
| Calculated MW | 113kDa |
| Observed MW | 145kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | A-431 treated by EGF |
| Cellular location | Cytoplasm, Preautophagosomal structure, cytosol. |
| Customer validation | WB(Spodoptera frugiperda, Homo sapiens, Rattus norvegicus, Fathead minnow, Oryctolagus cuniculus, Sus scrofa) IHC(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP0736? Please let us know so that we can cite the reference in this datasheet.
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