제품 > 항체 > 인산화 특이적 항체

Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (AP0297) at 1:2000 dilution or MAPK14 antibody (A14401). HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from NIH/3T3 cells using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from C6 cells using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297) at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review

Overview

Product name	Phospho-p38 MAPK-T180/Y182 Rabbit pAb
Catalog No.	AP0297
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T180 & Y182 of human p38 MAPK (NP_620581.1).
Sequence	MTGYV
Gene ID
Swiss Prot
Synonyms	RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Phospho-p38 MAPK-T180/Y182
Calculated MW	41kDa
Observed MW	41kDa/43 kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa cells treated by TNF-α, NIH/3T3 and C6 cells treated by UV, C6, NIH/3T3
Cellular location	Cytoplasm, Nucleus.
Customer validation	(mouse cells, HepG2) WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-p38 MAPK-T180/Y182 Rabbit pAb images

Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AP0297

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

VIRUSES-BASEL

Application:

IF:

5.048

Species:

Mus musculus

PMID:

26053925

Title:

Hydrogen Peroxide Induce Human Cytomegalovirus Replication through the Activation of p38-MAPK Signaling Pathway

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Brain research

Application:

IF:

3.252

Species:

Rattus norvegicus

PMID:

31075261

Title:

Upregulation of matrix metalloproteinase-9/2 in the wounded tissue, dorsal root ganglia, and spinal cord is involved in the development of postoperative pain.

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

BMC Molecular Biology

Application:

IF:

2.946

Species:

Homo sapiens

PMID:

31703613

Title:

PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

32589884

Title:

6H2L, a novel synthetic derivative of bifendate, induces apoptosis in hepatoma cells via mitochondrial and MAPK pathway

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

29207192

Title:

Resveratrol attenuates hydrogen peroxide‑induced apoptosis, reactive oxygen species generation, and PSGL‑1 and VWF activation in human umbilical vein endothelial cells, potentially via MAPK signalling pathways

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.996

Species:

Homo sapiens

PMID:

27586056

Title:

Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

J Inflamm Res

Application:

IF:

4.631

Species:

Homo sapiens, Mus musculus

PMID:

34239314

Title:

Cadherin-11 Deficiency Attenuates Ang-II-Induced Atrial Fibrosis and Susceptibility to Atrial Fibrillation

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Cancer Research and Treatment

Application:

IF:

5.036

Species:

Homo sapiens

PMID:

31048666

Title:

Cathepsin C Interacts with TNF-/p38 MAPK Signaling Pathway to Promote Proliferation and Metastasis in Hepatocellular Carcinoma.

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Atherosclerosis

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

38447436

Title:

Coptisine inhibits neointimal hyperplasia through attenuating Pak1/Pak2 signaling in vascular smooth muscle cells without retardation of re-endothelialization

References for Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0297)

Product:

Phospho-p38 MAPK-T180/Y182 Rabbit pAb

Journal:

Hypertension (Dallas, Tex. : 1979)

Application:

IF:

9.897

Species:

Rattus norvegicus

PMID:

29891648