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Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence analysis of Hep G2 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-p38 MAPK-Y182 Rabbit mAb
Catalog No.	AP1372
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58398

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y182 of human MAPK.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Phospho-p38 MAPK-Y182
Calculated MW	41kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Mus musculus)

Documents

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Research Area

Phospho-p38 MAPK-Y182 Rabbit mAb images

Immunohistochemistry - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence analysis of Hep G2 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence analysis of HeLa using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence analysis of NIH/3T3 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372)

Immunofluorescence analysis of PC-12 using Phospho-p38 MAPK-Y182 Rabbit mAb (AP1372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.