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Rig-I/DDX58 Rabbit pAb (A0550)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from HepG2 cells, using Rig-I/DDX58 Rabbit pAb (A0550) at 1:4000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from HeLa cells using Rig-I/DDX58 Rabbit pAb (A0550) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Review

Overview

Product name	Rig-I/DDX58 Rabbit pAb
Catalog No.	A0550
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 825-925 of human Rig-I/DDX58 (NP_055129.2).
Sequence	VIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK
Gene ID
Swiss Prot
Synonyms	RIG1; DDX58; RIG-I; RLR-1; SGMRT2; Rig-I/DDX58
Calculated MW	107kDa
Observed MW	107kDa/

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:4000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HepG2, HeLa
Cellular location	Cell junction, Cell projection, Cytoplasm, cytoskeleton, ruffle membrane, tight junction.
Customer validation	(T-47D、HepG2) WB(Homo sapiens, Sus scrofa, Mus musculus, Mus musculus) IF(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

연구면역학 & 염증톨-유사 수용체 신호전달 경로

Rig-I/DDX58 Rabbit pAb images

Western blot - Rig-I/DDX58 Rabbit pAb (A0550)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

26931521

Title:

Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Molecular therapy. Nucleic acids

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

30753994

Title:

The Downregulation of MicroRNA hsa-miR-340-5p in IAV-Infected A549 Cells Suppresses Viral Replication by Targeting RIG-I and OAS2.

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Veterinary Microbiology

Application:

IF:

2.4

Species:

Sus scrofa

PMID:

31648727

Title:

Japanese Encephalitis Virus infection induces inflammation of swine testis through RIG-I-NF-ĸB signaling pathway

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

PLoS Pathog

Application:

IF:

5.5

Species:

Mus musculus

PMID:

34529741

Title:

Histone demethylase LSD1 promotes RIG-I poly-ubiquitination and anti-viral gene expression

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

36032158

Title:

DEAD-box RNA helicase 21 negatively regulates cytosolic RNA-mediated innate immune signaling - PMC

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

5.5

Species:

Homo sapiens

PMID:

37956198

Title:

SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination - PMC

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

3.8

Species:

Homo sapiens, Mus musculus

PMID:

26892273

Title:

Amplified RLR signaling activation through an interferon-stimulated gene-endoplasmic reticulum stress-mitochondrial calcium uniporter protein loop

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

EMBO Reports

Application:

WB, IF

IF:

6.5

Species:

Homo sapiens

PMID:

27312109

Title:

RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling.

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

Species:

Sus scrofa

PMID:

38088561

Title:

Lactate-lactylation-HSPA6 axis promotes PRRSV replication by impairing IFN- production

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.6

Species:

Mus musculus

PMID:

37480121

Title:

H3K27me3 of Rnf19a promotes neuroinflammatory response during Japanese encephalitis virus infection - PMC

References for Rig-I/DDX58 Rabbit pAb (A0550)

Product:

Rig-I/DDX58 Rabbit pAb

Journal:

Journal for immunotherapy of cancer

Application:

IF:

10.9

Species:

Homo sapiens

PMID:

38749537

Title:

Original research: Colorectal cancer-specific IFN delivery overcomes dysfunctional dsRNA-mediated type I interferon signaling to increase the abscopal effect of radiotherapy - PMC