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SFPQ Rabbit mAb (A3494)

Review (1)Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SFPQ Rabbit mAb (A3494)

Western blot analysis of various lysates using SFPQ Rabbit mAb (A3494) at 1:200 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of C6 cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of HeLa cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of NIH-3T3 cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for SFPQ Rabbit mAb(A3494)

Overview

Product nameSFPQ Rabbit mAb
Catalog No.A3494
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0788
Enables DNA binding activity; histone deacetylase binding activity; and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin; nuclear matrix; and paraspeckles.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-707 of human SFPQ (P23246).
SequenceMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF
Gene ID
Swiss Prot
SynonymsPSF; POMP100; PPP1R140; SFPQ
Calculated MW76kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:50 - 1:200
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293F, HeLa
Cellular locationCytoplasm, Nucleus matrix, Nucleus speckle
Customer validation

(E14)

IF(Homo sapiens)

WB(Homo sapiens)

Documents

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ABclonal:Western blot - SFPQ Rabbit mAb (A3494)}

Western blot - SFPQ Rabbit mAb (A3494)

Western blot analysis of various lysates using SFPQ Rabbit mAb (A3494) at 1:200 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SFPQ Rabbit mAb (A3494)}

Immunohistochemistry - SFPQ Rabbit mAb (A3494)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using SFPQ Rabbit mAb (A3494) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)}

Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of C6 cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)}

Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of HeLa cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SFPQ Rabbit mAb (A3494)}

Immunofluorescence - SFPQ Rabbit mAb (A3494)

Immunofluorescence analysis of NIH-3T3 cells using SFPQ Rabbit mAb (A3494) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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