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TTC11/FIS1 Rabbit pAb (A5821)

Reviews (2)Publications (17) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TTC11/FIS1 Rabbit pAb (A5821)

Western blot analysis of lysates from 293T cells, using TTC11/FIS1 Rabbit pAb (A5821) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TTC11/FIS1 Rabbit pAb (A5821)

Confocal immunofluorescence analysis of HeLa cells using TTC11/FIS1 Rabbit pAb (A5821) at dilution of 1:50 (60x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - TTC11/FIS1 Rabbit pAb (A5821)

Immunoprecipitation analysis of 200 μg extracts of MCF7 cells using 3 μg TTC11/FIS1 antibody (A5821). Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody (A5821) at a dilution of 1:1000.

Review
ABclonal: review for TTC11/FIS1 Rabbit pAb(A5821)
Review
ABclonal: review for TTC11/FIS1 Rabbit pAb(A5821)

Overview

Product nameTTC11/FIS1 Rabbit pAb
Catalog No.A5821
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cellular calcium ion homeostasis; and mitochondrion organization. Acts upstream of or within mitochondrion morphogenesis. Located in mitochondrion and peroxisome. Is integral component of mitochondrial outer membrane and integral component of peroxisomal membrane. Part of protein-containing complex. Biomarker of Alzheimer's disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11/FIS1 (NP_057152.2).
SequenceMEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDG
Gene ID
Swiss Prot
SynonymsTTC11; CGI-135; TTC11/FIS1
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7
Cellular locationMitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein.
Customer validation

(Human bone marrow cancer cell)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus)

IP(Homo sapiens, Mus musculus)

Co-IP(Mus musculus)

IF(Mus musculus)

WB(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - TTC11/FIS1 Rabbit pAb (A5821)}

Western blot - TTC11/FIS1 Rabbit pAb (A5821)

Western blot analysis of lysates from 293T cells, using TTC11/FIS1 Rabbit pAb (A5821) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - TTC11/FIS1 Rabbit pAb (A5821)}

Immunofluorescence - TTC11/FIS1 Rabbit pAb (A5821)

Confocal immunofluorescence analysis of HeLa cells using TTC11/FIS1 Rabbit pAb (A5821) at dilution of 1:50 (60x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - TTC11/FIS1 Rabbit pAb (A5821)}

Immunoprecipitation - TTC11/FIS1 Rabbit pAb (A5821)

Immunoprecipitation analysis of 200 μg extracts of MCF7 cells using 3 μg TTC11/FIS1 antibody (A5821). Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody (A5821) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)