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[KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and TTC11/FIS1 knockout (KO) HeLa(KO) cells, using [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates, using [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Confocal imaging of U-2 OS cells using [KO Validated] TTC11/FIS1 Rabbit mAb (A19666, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

ABclonal:Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunoprecipitation of TTC11/FIS1 from 300 µg extracts of HeLa cells was performed using 3 µg of TTC11/FIS1 antibody (A19666). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TTC11/FIS1 (A19666) at a dilution of 1:2000.

Overview

Product name	[KO Validated] TTC11/FIS1 Rabbit mAb
Catalog No.	A19666
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5010-03

Background

Enables identical protein binding activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cellular calcium ion homeostasis; and mitochondrion organization. Acts upstream of or within mitochondrion morphogenesis. Located in mitochondrion and peroxisome. Is integral component of mitochondrial outer membrane and integral component of peroxisomal membrane. Part of protein-containing complex. Biomarker of Alzheimer's disease.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11/FIS1 (NP_057152.2).
Sequence	MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDG
Gene ID
Swiss Prot
Synonyms	TTC11; CGI-135; S1
Calculated MW	17kDa
Observed MW	15kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:800 IF/ICC 1:100 - 1:400 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, HepG2, Mouse heart, Rat spleen, Rat brain
Cellular location	Mitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein.
Customer validation	WB(Mus musculus, Rattus norvegicus, Sus scrofa, Homo sapiens, Rattus norvegicus) IHC(Mus musculus, Rattus norvegicus) IF(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구내분비 & 대사미토콘드리아 대사미토콘드리아 마커

[KO Validated] TTC11/FIS1 Rabbit mAb images

Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Arch Biochem Biophys

Application:

IF:

4.013

Species:

Mus musculus

PMID:

34153296

Title:

Glucose fluctuation accelerates cardiac injury of diabetic mice via sodium-dependent glucose cotransporter 1 (SGLT1)

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Food Funct

Application:

IF:

5.396

Species:

Mus musculus

PMID:

34821902

Title:

Ganoderic acid improves 5-fluorouracil-induced cognitive dysfunction in mice

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Biochemistry and cell biology = Biochimie et biologie cellulaire

Application:

IF:

3.73

Species:

Rattus norvegicus

PMID:

33259229

Title:

Knockdown of SGLT1 prevents the apoptosis of cardiomyocytes induced by glucose fluctuation via relieving oxidative stress and mitochondrial dysfunction

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

3.32

Species:

Mus musculus

PMID:

35447394

Title:

Grpel2 alleviates myocardial ischemia/reperfusion injury by inhibiting MCU-mediated mitochondrial calcium overload

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Behavioural brain research

Application:

IF:

3.35

Species:

Mus musculus

PMID:

36535434

Title:

Two polyphenols isolated from Corallodiscus flabellata BL Burtt ameliorate amyloid β-protein induced Alzheimer's disease neuronal injury by improving mitochondrial …

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Sus scrofa

PMID:

37031286

Title:

The apoptotic effects of soybean agglutinin were induced through three different signal pathways by down-regulating cytoskeleton proteins in IPEC-J2 cells - PMC

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

37488119

Title:

Effects of oxidative stress on hepatic encephalopathy pathogenesis in mice - PMC

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Oncogene

Application:

IF:

8.75

Species:

Homo sapiens

PMID:

36759571

Title:

Retrograde regulation of mitochondrial fission and epithelial to mesenchymal transition in hepatocellular carcinoma by GCN5L1

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.29

Species:

Rattus norvegicus

PMID:

37593898

Title:

Endoplasmic reticulum stress induced by turbulence of mitochondrial fusion and fission was involved in stressed cardiomyocyte injury

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

WB, IHC, IF

IF:

7.67

Species:

Mus musculus, Rattus norvegicus

PMID:

37237889

Title:

Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress - PMC

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Mus musculus

PMID:

37108341

Title:

Defective Mitochondrial Dynamics and Protein Degradation Pathways Underlie Cadmium-Induced Neurotoxicity and Cell Death in Huntingtons Disease Striatal Cells - PMC

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Journal of molecular and cellular cardiology

Application:

IF:

5.76

Species:

Mus musculus

PMID:

36509022

Title:

Klf4 deficiency exacerbates myocardial ischemia/reperfusion injury in mice via enhancing ROCK1/DRP1 pathway-dependent mitochondrial fission

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Homo sapiens

PMID:

38539195

Title:

Human periodontal ligament stem cell sheets activated by graphene oxide quantum dots repair periodontal bone defects by promoting mitochondrial

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

38417794

Title:

ORMDL3 regulates NLRP3 inflammasome activation by maintaining ER-mitochondria contacts in human macrophages and dictates ulcerative colitis patient outcome - PMC

References for [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Product:

[KO Validated] TTC11/FIS1 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Rattus norvegicus

PMID:

38724553