제품 > 항체 > 단일클론항체(mAb)

TUG/ASPSCR1 Rabbit mAb (A1396)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using TUG/ASPSCR1 Rabbit mAb (A1396) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	TUG/ASPSCR1 Rabbit mAb
Catalog No.	A1396
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2566

Background

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human TUG/ASPSCR1 (Q9BZE9).
Sequence	LPLESGELSRGDLSRPEDADTSGPCCEHTQEKQSTRAPAAAPFVPFSGGGQRLGGPPGPTRPLTSSSAKLPKSLSSPGGPSKPKKSKSGQDPQQEQEQERE
Gene ID
Swiss Prot
Synonyms	TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1; TUG/ASPSCR1
Calculated MW	60kDa
Observed MW	75kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T, NIH/3T3, RD, Mouse testis
Cellular location	Cytoplasm, Endomembrane system, Endoplasmic reticulum-Golgi intermediate compartment membrane, Nucleus, Peripheral membrane protein.