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cIAP2 Rabbit pAb (A0833)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of various lysates using cIAP2 Rabbit pAb (A0833) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using cIAP2 Rabbit pAb (A0833) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of A-549 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namecIAP2 Rabbit pAb
Catalog No.A0833
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-130 of human cIAP2 (NP_001156.1).
SequenceNSVNNLEATSQPTFPSSVTNSTHSLLPGTEN
Gene ID
Swiss Prot
SynonymsAIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; RNF49; c-IAP2; cIAP2
Calculated MW68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, RAW264.7
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - cIAP2 Rabbit pAb (A0833)}

Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of various lysates using cIAP2 Rabbit pAb (A0833) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833)}

Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using cIAP2 Rabbit pAb (A0833) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)}

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of A-549 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)}

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0833? Please let us know so that we can cite the reference in this datasheet.