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cIAP2 Rabbit pAb (A0833)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of various lysates using cIAP2 Rabbit pAb (A0833) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using cIAP2 Rabbit pAb (A0833) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of A-549 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namecIAP2 Rabbit pAb
Catalog No.A0833
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-130 of human cIAP2 (NP_001156.1).
SequenceNSVNNLEATSQPTFPSSVTNSTHSLLPGTEN
Gene ID
Swiss Prot
SynonymsAIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; RNF49; c-IAP2; cIAP2
Calculated MW68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, RAW264.7
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - cIAP2 Rabbit pAb (A0833)}

Western blot - cIAP2 Rabbit pAb (A0833)

Western blot analysis of various lysates using cIAP2 Rabbit pAb (A0833) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - cIAP2 Rabbit pAb (A0833)}

Immunohistochemistry - cIAP2 Rabbit pAb (A0833)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using cIAP2 Rabbit pAb (A0833) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)}

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of A-549 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - cIAP2 Rabbit pAb (A0833)}

Immunofluorescence - cIAP2 Rabbit pAb (A0833)

Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (A0833) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0833? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)