제품 > 항체 > 다중클론항체(pAb)

PSD95 Rabbit pAb (A6194)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using PSD95 antibody (A6194) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - PSD95 Rabbit pAb (A6194)

Immunofluorescence analysis of SH-SY5Y cells using PSD95 Rabbit pAb (A6194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	PSD95 Rabbit pAb
Catalog No.	A6194
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 420-510 of human PSD95 (NP_001308004.1).
Sequence	TASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSS
Gene ID
Swiss Prot
Synonyms	MRD62; PSD95; SAP90; SAP-90
Calculated MW	80kDa
Observed MW	95kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse brain, Rat brain
Cellular location	Cell junction, Cell membrane, Cell projection, Peripheral membrane protein, axon, postsynaptic cell membrane, postsynaptic density, synapse
Customer validation	WB(Mus musculus, Mus musculus , Homo sapiens, Rattus norvegicus) IF(Mus musculus) IF(Mus musculus) Other(Rattus norvegicus)

Documents

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Research Area

PSD95 Rabbit pAb images

Western blot - PSD95 Rabbit pAb (A6194)

Immunofluorescence - PSD95 Rabbit pAb (A6194)

Immunofluorescence analysis of SH-SY5Y cells using PSD95 Rabbit pAb (A6194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

36446229

Title:

ChemR23 signaling ameliorates cognitive impairments in diabetic mice via dampening oxidative stress and NLRP3 inflammasome activation - PMC

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

5.27

Species:

Mus musculus

PMID:

36396078

Title:

Impact of prenatal amoxicillin exposure on hippocampal development deficiency

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Mus musculus

PMID:

37130475

Title:

Exposure to polystyrene particles causes anxiety-, depression-like behavior and abnormal social behavior in mice

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

Homo sapiens

PMID:

37516664

Title:

Impact of Prenatal Acetaminophen Exposure for Hippocampal Development Disorder on Mice

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

37636073

Title:

Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice - PMC

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Life sciences

Application:

IF:

6.78

Species:

Mus musculus

PMID:

37634815

Title:

Aerobic exercise regulates GPR81 signal pathway and mediates complement-microglia axis homeostasis on synaptic protection in the early stage of Alzheimer's

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.682

Species:

Mus musculus

PMID:

28058580

Title:

Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Aging Cell

Application:

WB, IF

IF:

11.005

Species:

Mus musculus

PMID:

34363729

Title:

MeCP2 prevents age-associated cognitive decline via restoring synaptic plasticity in a senescence-accelerated mouse model

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

35697184

Title:

Silver nanoparticles induced hippocampal neuronal damage involved in mitophagy, mitochondrial biogenesis and synaptic degeneration

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38676295

Title:

Peripheral inflammation triggering central anxiety through the hippocampal glutamate metabolized receptor 1 - PMC

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

Journal of pharmaceutical analysis

Application:

IF:

8.8

Species:

Mus musculus

PMID:

38618251

Title:

Altered synaptic currents, mitophagy, mitochondrial dynamics in Alzheimer's disease models and therapeutic potential of Dengzhan Shengmai Capsules

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

38941212

Title:

Tolllike receptor 4dependent innate immune responses are mediated by intracrine corticosteroids and activation of glycogen synthase kinase3 in astrocytes

References for PSD95 Rabbit pAb (A6194)

Product:

PSD95 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

Other

IF:

4.8

Species:

Rattus norvegicus

PMID:

38941212

Title:

Tolllike receptor 4dependent innate immune responses are mediated by intracrine corticosteroids and activation of glycogen synthase kinase3 in astrocytes