제품 > 항체 > 단일클론항체(mAb)

eIF4EBP1 Rabbit mAb (A19045)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using eIF4EBP1 Rabbit mAb (A19045) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - eIF4EBP1 Rabbit mAb (A19045)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using eIF4EBP1 Rabbit mAb (A19045) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	eIF4EBP1 Rabbit mAb
Catalog No.	A19045
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0374

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4EBP1 (Q13541).
Sequence	MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRN
Gene ID
Swiss Prot
Synonyms	BP-1; 4EBP1; 4E-BP1; PHAS-I; eIF4EBP1
Calculated MW	13kDa
Observed MW	18kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	U-251MG, Rat skeletal muscle
Cellular location	cytoplasm, cytosol.
Customer validation	WB(Mus musculus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Research Area

eIF4EBP1 Rabbit mAb images

Western blot - eIF4EBP1 Rabbit mAb (A19045)

Immunohistochemistry - eIF4EBP1 Rabbit mAb (A19045)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Acta Pharm Sin B

Application:

IF:

14.7

Species:

Mus musculus

PMID:

34900535

Title:

Discovery of a potent FKBP38 agonist that ameliorates HFD-induced hyperlipidemia via mTOR/P70S6K/SREBPs pathway

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Oncol Lett

Application:

IF:

2.5

Species:

Mus musculus

PMID:

34267822

Title:

HSP60 knockdown suppresses proliferation in colorectal cancer cells via activating the adenine/AMPK/mTOR signaling pathway

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

35761332

Title:

Delivery of engineered extracellular vesicles with miR-29b editing system for muscle atrophy therapy - PMC

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Molecular therapy. Nucleic acids

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

36891498

Title:

Identification of a novel small-molecule inhibitor of miR-29b attenuates muscle atrophy - PMC

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

37088818

Title:

CircTmeff1 Promotes Muscle Atrophy by Interacting with TDP‐43 and Encoding A Novel TMEFF1‐339aa Protein

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

2.8

Species:

Homo sapiens

PMID:

37934902

Title:

Maackiain suppresses the development of cervical cancer via AMPK priming autophagy

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Journal of extracellular vesicles

Application:

IF:

Species:

Homo sapiens, Mus musculus

PMID:

35524442

Title:

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

36966152

Title:

Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma - PMC

References for eIF4EBP1 Rabbit mAb (A19045)

Product:

eIF4EBP1 Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

7.6

Species:

Homo sapiens

PMID:

37967710

Title:

Environment relevant exposure of perfluorooctanoic acid accelerates the growth of hepatocellular carcinoma cells through mammalian target of rapamycin (mTOR)