제품 > 항체 > 다중클론항체(pAb)

p53 Rabbit pAb (A11232)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Rat brain using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using p53 Rabbit pAb (A11232) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Review

Overview

Product name	p53 Rabbit pAb
Catalog No.	A11232
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 95-390 of mouse p53 (NP_035770.2).
Sequence	PSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD
Gene ID
Swiss Prot
Synonyms	bbl; bfy; bhy; p44; p53; Tp53
Calculated MW	43kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Customer validation	(rat cells) WB(Homo sapiens, Mus musculus, Ovis aries, Rattus norvegicus) IHC(Rattus norvegicus) IHC(Rattus norvegicus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

p53 Rabbit pAb images

Immunohistochemistry - p53 Rabbit pAb (A11232)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

Oncology Reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

32901874

Title:

Anti‑proliferative effect of honokiol on SW620 cells through upregulating BMP7 expression via the TGF‑β1/p53 signaling pathway

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

Oncol Lett

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

33240422

Title:

TERT promoter regulating melittin expression induces apoptosis and G 0/G 1 cell cycle arrest in esophageal carcinoma cells

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

IntImmunopharmacol

Application:

IHC

IF:

4.8

Species:

Rattus norvegicus

PMID:

34217992

Title:

Betulin alleviates cisplatin-induced hepatic injury in rats: Targeting apoptosis and Nek7-independent NLRP3 inflammasome pathways

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

37610095

Title:

Acid ground nanorealgar processed product inhibits breast cancer by inducing mitophagy via the p53/BNIP3/NIX pathway

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

Cellular & molecular biology letters

Application:

IF:

9.2

Species:

Mus musculus

PMID:

38093179

Title:

Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degeneration - PMC

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Ovis aries

PMID:

38534401

Title:

MiR-23b and miR-133 cotarget TGF2/NOTCH1 in dermal fibroblasts and affect hair follicle development in sheep

References for p53 Rabbit pAb (A11232)

Product:

p53 Rabbit pAb

Journal:

PeerJ

Application:

WB, IHC, IF

IF:

2.3

Species:

Rattus norvegicus

PMID:

35935259

Title:

Advanced oxidation protein products induce annulus fibrosus cell senescence through a NOX4-dependent, MAPK-mediated pathway and accelerate intervertebral disc degeneration - PMC