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ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)

Flow cytometry: 1X10^6 Daudi cells (negative control, left) and HeLa (right) were surface-stained with ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Daudi cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)

Flow cytometry:1X10^6 HeLa were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human β2 Microglobulin mAb
Catalog No.A24223
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60950-ABflo488
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1).
SequenceIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Gene ID
Swiss Prot
SynonymsB2M; IMD43; beta-2-microglobulin
Calculated MW13kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationSecreted, Cell surface

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)

Flow cytometry: 1X10^6 Daudi cells (negative control, left) and HeLa (right) were surface-stained with ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Daudi cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)

Flow cytometry:1X10^6 HeLa were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (9)

ELISA 키트 (1)

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Secondary Antibodies (26)