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beta 2 Microglobulin Rabbit pAb (A12404)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - beta 2 Microglobulin Rabbit pAb (A12404)

Western blot analysis of various lysates, using beta 2 Microglobulin Rabbit pAb (A12404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded human liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded mouse liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded rat liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namebeta 2 Microglobulin Rabbit pAb
Catalog No.A12404
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
SequenceIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Gene ID
Swiss Prot
SynonymsIMD43; beta 2 Microglobulin
Calculated MW14kDa
Observed MW12kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse liver, Rat liver, Mouse lung
Cellular locationSecreted
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - beta 2 Microglobulin Rabbit pAb (A12404)}

Western blot - beta 2 Microglobulin Rabbit pAb (A12404)

Western blot analysis of various lysates, using beta 2 Microglobulin Rabbit pAb (A12404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)}

Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded human liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)}

Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded mouse liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)}

Immunohistochemistry - beta 2 Microglobulin Rabbit pAb (A12404)

Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded rat liver using beta 2 Microglobulin Rabbit pAb (A12404) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (9)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)