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[KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and β2-microglobulin knockdown (KD) HeLa cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Immunofluorescence analysis of HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Flow cytometry:1X10^6 Daudi cells(negative control, left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(A23430, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line).Non-fluorescently stained cells was used as blank control (red line).

Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or β2 Microglobulin Rabbit mAb(A23430, 2 μg/mL, right).

Overview

Product name	[KD Validated] beta 2 Microglobulin Rabbit mAb
Catalog No.	A23430
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60950

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1).
Sequence	IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Gene ID
Swiss Prot
Synonyms	B2M; IMD43; beta-2-microglobulin; [KD Validated] beta 2 Microglobulin
Calculated MW	13kDa
Observed MW	12kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	THP-1, HeLa, U-937
Cellular location	Secreted, Cell surface

Documents

Certificate of Compliance

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Research Area

[KD Validated] beta 2 Microglobulin Rabbit mAb images

Western blot - [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Immunofluorescence - [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Flow CytoMetry - [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)

Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or β2 Microglobulin Rabbit mAb(A23430, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.