제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human β2 Microglobulin mAb (A24224)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 Daudi cells(negative control, left) and HeLa (right) were surface-stained with ABflo® 647 Rabbit anti-Human β2 Microglobulin mAb(A24224, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 HeLa were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human β2 Microglobulin mAb(A24224, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human β2 Microglobulin mAb
Catalog No.	A24224
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60950-ABflo647

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1).
Sequence	IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Gene ID
Swiss Prot
Synonyms	B2M; IMD43; beta-2-microglobulin
Calculated MW	13kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Secreted, Cell surface