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ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)

Flow cytometry:1X10^6 MCF7 cells (negative control, Left) and HEL cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb(A22586, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)

Flow cytometry:1X10^6 HEL cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb(A22586, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human CD117/c-Kit mAb
Catalog No.A22586
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56005-ABf488
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-520 of human CD117/c-Kit (NP_000213.1).
SequenceQPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHPHT
Gene ID
Swiss Prot
SynonymsPBT; SCFR; C-Kit; CD117; MASTC
Calculated MW110kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCytoplasmic side of plasma membrane, External side of plasma membrane, Extracellular space, Fibrillar center, Plasma membrane

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)

Flow cytometry:1X10^6 MCF7 cells (negative control, Left) and HEL cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb(A22586, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb (A22586)

Flow cytometry:1X10^6 HEL cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD117/c-Kit mAb(A22586, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (8)

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Secondary Antibodies (26)