제품 > 항체 > 인산화 특이적 항체

Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385)

Western blot analysis of lysates from HeLa cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-CD117/c-Kit-Y721 Rabbit pAb
Catalog No.AP0385
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y721 of human KIT
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsPBT; SCFR; C-Kit; CD117; MASTC; Phospho-CD117/c-Kit-Y721
Calculated MW110kDa
Observed MW130kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationCell membrane, Cytoplasm, Single-pass type I membrane protein
ABclonal:Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385)}

Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385)

Western blot analysis of lysates from HeLa cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0385).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (8)

재조합 단백질 (3)

Secondary Antibodies (26)